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Published on: October 12, 2018
Cytogenetic changes in oral mucosa cells from individuals submitted to oral human immunodeficiency virus pre-exposure
Maria Esther Suarez Alpire1, Daniel Vitor de Souza1, Carolina Marquez da Costa Brites Masutti2
1Universidade Federal de São Paulo, Instituto de Saúde e Sociedade, Departamento de Biociências - Santos (SP), Brazil.
Objective:
The objective of this study was to evaluate cytogenetic changes in individuals submitted to oral human immunodeficiency virus pre-exposure prophylaxis use through the micronucleus test in oral mucosa.
Methods:
This study consisted of 37 individuals, of whom 17 comprised the pre-exposure prophylaxis group and 20 comprised the control group. A total of 2,000 cells per slide were analyzed for the determination of micronuclei, binucleation, nuclear buds, and cytotoxicity parameters: pyknosis, karyolysis, and karyorrhexis (KR), in a double-blind manner. The repair index was also evaluated in this setting.
Results:
In the mutagenicity parameters, the pre-exposure prophylaxis group showed increased frequencies of micronuclei (p=0.0001), binucleation (p=0.001), and nuclear buds (p=0.07). Regarding the cytotoxicity parameters, there was an increase with a statistical difference (p≤0.05) in the karyorrhexis frequency (p=0.001). Additionally, the repair system efficiency decreased in the pre-exposure prophylaxis group.
Conclusion:
These results indicate that individuals undergoing pre-exposure prophylaxis use have geno- and cytotoxicity in oral mucosal cells.
