Duodenal-Jejunal bypass improves metabolism and re-models extra cellular matrix through modulating ceRNA network

Area of Science:

• Metabolic Surgery
• Obesity Research
• RNA Biology

Background:

• Bariatric surgery (BS), including duodenal-jejunal bypass (DJB), effectively treats obesity and type 2 diabetes mellitus (T2DM).
• Previous research indicated DJB alters gastrointestinal long non-coding RNAs (lncRNAs), influencing lipid metabolism and glycemic control via gut-brain and entero-pancreatic axes.
• The impact of DJB on adipose tissue non-coding RNA expression profiles and competing endogenous RNA (ceRNA) networks requires further investigation.

Purpose of the Study:

• To compare lncRNA, circular RNA (circRNA), and messenger RNA (mRNA) expression in adipose tissue after DJB.
• To investigate the ceRNA regulatory networks influenced by DJB in adipose tissue.
• To elucidate the molecular mechanisms underlying DJB's effects on metabolism and insulin sensitivity.

Main Methods:

• Differential expression analysis of lncRNAs, circRNAs, and mRNAs in adipose tissue from sham and DJB groups.
• Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of differentially expressed mRNAs (DEmRNAs).
• Construction and analysis of ceRNA regulatory networks involving DElncRNAs, DEcircRNAs, and DEmRNAs.

Main Results:

• Identified 2219 DEmRNAs, 722 DElncRNAs, and 425 DEcircRNAs.
• DEmRNAs were associated with lipid metabolism, amino acid metabolism, insulin resistance, and extracellular matrix (ECM) pathways.
• Analysis revealed 31 hub DE mRNAs within ceRNA networks, including Mpp7, 9330159F19Rik, Trhde, Trdn, and Sorbs2.

Conclusions:

• DJB influences adipose tissue remodeling, particularly the ECM.
• The observed metabolic improvements are mediated through ceRNA regulatory networks.
• DJB's effects on energy metabolism are linked to altered non-coding RNA expression and subsequent pathway modulation.