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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

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Published on: March 13, 2018

Advancing Multiple Detection in RT-LAMP with a Specific Probe Assembled from Plural Three-Way-Junction Structures.

Yi Sun^1,2, Sicai Zhang^1,3, Lijuan Qi^1,2

¹State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.

Analytical Chemistry

|November 16, 2023

Summary

This study introduces a novel multivariate real-time fluorescent probe (MRTFP) for rapid Salmonella detection. MRTFP enables sensitive, specific, and simultaneous identification of multiple Salmonella strains in food samples.

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Last Updated: Jul 11, 2025

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Published on: March 13, 2018

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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

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Published on: October 23, 2011

Area of Science:

Molecular Biology
Diagnostic Microbiology
Biotechnology

Background:

Accurate pathogen identification is crucial for disease control.
Existing diagnostic methods often lack speed, sensitivity, or multiplexing capabilities.

Purpose of the Study:

To develop a novel, highly sensitive, and specific multivariate probe for pathogen detection.
To enable rapid, single-step, multiplexed detection of Salmonella using real-time loop-mediated isothermal amplification (RT-LAMP).

Main Methods:

Assembly of strand exchange three-way-junction (3WJ) structures into four-angle probes (FP) and hexagonal probes (HP).
Incorporation of FPs and HPs into RT-LAMP assays for Salmonella gene targeting.
Testing of the multivariate real-time fluorescent probe (MRTFP) on real food samples.

Main Results:

The developed MRTFP achieved sensitive and specific single-step, multiplexed detection of Salmonella in RT-LAMP.
Successfully identified and distinguished Salmonella enteritidis and Salmonella typhimurium in food samples with a minimum detectable concentration of 10^3 CFU/mL.
Demonstrated simultaneous detection of dual or triple targets in a single tube, enhancing detection efficiency.

Conclusions:

MRTFP offers a simplified design for robust multivariate probes with excellent stability and no cross-interference.
This technology holds significant potential for developing efficient and accurate diagnostic tools for disease detection and pathogen identification.