Related Experiment Video
Updated: Jul 11, 2025

Fear Incubation Using an Extended Fear-Conditioning Protocol for Rats
Published on: August 22, 2020
Infant gut microbiota and negative and fear reactivity
Venla Huovinen1, Anna-Katariina Aatsinki1,2, Eeva-Leena Kataja1
1Department of Clinical Medicine, Psychiatry, FinnBrain Birth Cohort Study, Turku Brain and Mind Center, University of Turku, Turku, Finland.
Background:
Studies indicate that gut microbiota is related to neurodevelopmental and behavioral outcomes. Accordingly, early gut microbiota composition (GMC) has been linked to child temperament, but research is still scarce. The aim of this study was to examine how early GMC at 2.5 months is associated with child negative and fear reactivity at 8 and 12 months since they are potentially important intermediate phenotypes of later child psychiatric disorders.
Methods:
Our study population was 330 infants enrolled in the longitudinal FinnBrain Birth Cohort Study. Gut microbiota composition was analyzed using stool sample 16s rRNA sequencing. Negative and fear reactivity were assessed using the Laboratory Temperament Assessment Battery (Lab-TAB) at child's age of 8 months (n =150) and the Infant Behavior Questionnaire-Revised Short Form (IBQ-R SF) at child's age of 12 months (n = 276).
Conclusions:
We found a positive association between alpha diversity and reported fear reactivity and differing microbial community composition based on negative reactivity for boys. Isobutyric acid correlated with observed negative reactivity, however, this association attenuated in the linear model. Several genera were associated with the selected infant temperament traits. This study adds to the growing literature on links between infant gut microbiota and temperament informing future mechanistic studies.
Related Concept Videos
Preparedness and Phobias
Socioemotional Development during Infancy
Primary Temperament Types
Piaget's Stage 1 of Cognitive Development
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Attachment
Development of Immunocompetence
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...

