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Updated: Jun 27, 2026

Assays for the Identification of Novel Antivirals against Bluetongue Virus
Published on: October 11, 2013
Antiviral evaluation of 1,4-disubstituted-1,2,3-triazole derivatives against Chikungunya virus
Vitor Won-Held Rabelo1, Verônica Diniz da Silva2, Maria Leonisa Sanchez Nuñez1
1Programa de Pós-graduação em Ciências e Biotecnologia, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ, CEP, 24210-201, Brazil.
Aim:
This work aimed to investigate the antiviral activity of two 1,4-disubstituted-1,2,3-triazole derivatives (1 and 2) against Chikungunya virus (CHIKV) replication.
Materials & Methods:
Cytotoxicity was analyzed using colorimetric assays and the antiviral potential was evaluated using plaque assays and computational tools.
Results:
Compound 2 showed antiviral activity against CHIKV 181-25 in BHK-21 and Vero cells. Also, this compound presented a higher activity against CHIKV BRA/RJ/18 in Vero cells, like compound 1. Compound 2 exhibited virucidal activity and inhibited virus entry while compound 1 inhibited virus release. Molecular docking suggested that these derivatives inhibit nsP1 protein while compound 1 may also target capsid protein.
Conclusion:
Both compounds exhibit promising antiviral activity against CHIKV by blocking different steps of virus replication.
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