Resolving D-Amino Acid Containing Peptides Using Ion Mobility-Mass Spectrometry: Challenges and Recent Developments
Mohamed Abdulbagi1, Bin Di1,2,3, Bo Li1,2,3
1Center Key Laboratory on Protein Chemistry and Structural Biology, China Pharmaceutical University, Nanjing, China.
Critical Reviews in Analytical Chemistry
|November 17, 2023
Summary
D-amino acid peptides are vital in biology and medicine. Ion mobility mass spectrometry (IM-MS) offers a powerful new method for detecting and analyzing these D-amino acid containing peptides (DAACPs).
Area of Science:
- Biochemistry
- Analytical Chemistry
- Proteomics
Background:
- D-amino acids are increasingly recognized as widespread in biological systems.
- Peptides containing D-amino acids (DAACPs) possess diverse biological functions and therapeutic applications.
- Discovering and analyzing DAACPs presents significant challenges.
Purpose of the Study:
- To review the significance and challenges associated with D-amino acid containing peptides (DAACPs).
- To highlight the advancements in Ion Mobility Mass Spectrometry (IM-MS) for DAACP detection and analysis.
- To explore the potential of LC-IM-MS/MS for isomeric mixture analysis.
Main Methods:
- Utilizing Ion Mobility Mass Spectrometry (IM-MS) for separation based on ion shape and mobility.
- Analyzing fragment ion arrival times to precisely locate D-amino acid positions.
- Employing a Liquid Chromatography-Ion Mobility-Mass Spectrometry/Mass Spectrometry (LC-IM-MS/MS) platform.
Main Results:
- IM-MS provides baseline separation of peptides with D-amino acids versus L-amino acids due to differences in collision cross-section.
- IM-MS enables precise localization of D-amino acid residues within peptide sequences.
- Recent IM-MS developments have significantly improved the detection and analysis of DAACPs.
Conclusions:
- D-amino acid containing peptides are important molecules with diverse biological roles.
- Ion mobility mass spectrometry is a key technology for overcoming challenges in DAACP discovery and characterization.
- The LC-IM-MS/MS platform shows great promise for analyzing complex isomeric mixtures of peptides.
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