Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fabricated references are twice as common in medRxiv preprints as in peer-reviewed articles.

Journal of internal medicine·2026
Same author

Integrating diversity, equity, and inclusion in generative AI applications for healthcare education: a scoping review.

International journal of medical informatics·2026
Same author

Generation of two CASK patient-derived human induced pluripotent stem cell lines to study CASK-related disorders.

Stem cell research·2026
Same author

Synaptosomes isolated from cryopreserved MND motor cortex reveal altered calcium handling and reduced complex IV-linked respiration.

Frontiers in synaptic neuroscience·2026
Same author

Size-dependent alterations in phrenic motor neuron perineuronal nets following cervical spinal hemisection.

Journal of neurophysiology·2026
Same author

Environmental Antibiotic Contamination and AMR: Integrating Pathways, Impacts, and AI-Driven Mitigation.

Environmental toxicology and chemistry·2026

Related Experiment Video

Updated: Jul 11, 2025

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

8.9K

Protocol for generating embedding-free brain organoids enriched with oligodendrocytes.

Bahaa Al-Mhanawi1, Marta Boira Marti1, Sean D Morrison1

  • 1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia.

STAR Protocols
|November 17, 2023
PubMed
Summary

Researchers developed a new protocol for creating human brain organoids rich in myelin-producing cells. These advanced in vitro models are crucial for studying white matter diseases like leukodystrophy.

Keywords:
NeuroscienceOrganoidsStem Cells

More Related Videos

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
05:40

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Published on: May 5, 2022

3.8K
Generation of Human Brain Organoids for Mitochondrial Disease Modeling
08:09

Generation of Human Brain Organoids for Mitochondrial Disease Modeling

Published on: June 21, 2021

6.2K

Related Experiment Videos

Last Updated: Jul 11, 2025

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
08:30

A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells

Published on: March 4, 2020

8.9K
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
05:40

Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Published on: May 5, 2022

3.8K
Generation of Human Brain Organoids for Mitochondrial Disease Modeling
08:09

Generation of Human Brain Organoids for Mitochondrial Disease Modeling

Published on: June 21, 2021

6.2K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Biomedical Engineering

Background:

  • Limited in vitro models exist for human central nervous system (CNS) white matter research.
  • Oligodendrocytes, responsible for myelination, are critical components of white matter.

Purpose of the Study:

  • To present a protocol for generating advanced, embedding-free human brain organoids.
  • To enrich these organoids with oligodendrocytes for white matter research.

Main Methods:

  • Neuroectoderm differentiation and neural spheroid development.
  • Transferal of spheroids to Matrigel for organoid formation.
  • Maturation and application of oligodendrocyte-enriched brain organoids.

Main Results:

  • Successful generation of human brain organoids enriched with myelin-producing cells (oligodendrocytes).
  • Demonstrated utility of these organoids for studying myelination processes.

Conclusions:

  • The developed protocol provides a valuable in vitro model for human white matter research.
  • These organoids are suitable for investigating white matter diseases, including leukodystrophy.