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Updated: Jul 11, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Simultaneous deep transcriptome and proteome profiling in a single mouse oocyte.

Yi-Rong Jiang¹, Le Zhu², Lan-Rui Cao²

¹Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310058, China.

|November 17, 2023

Summary

This summary is machine-generated.

A new platform enables simultaneous single-cell transcriptome and proteome analysis. This breakthrough offers deep insights into gene and protein expression, aiding the study of cellular processes like oocyte meiosis.

Keywords:

CP: Developmental biology CP: Molecular biology RNA sequencing microfluidics oocyte meiotic maturation shotgun proteomics single-cell multi-omics

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Last Updated: Jul 11, 2025

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

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Published on: June 16, 2021

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

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Published on: February 24, 2023

Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes

Use of Single Molecule Fluorescent In Situ Hybridization SM-FISH to Quantify and Localize mRNAs in Murine Oocytes

Published on: April 24, 2019

Area of Science:

Single-cell biology
Molecular biology
Genomics and Proteomics

Background:

Simultaneous transcriptome and proteome analysis at the single-cell level presents significant technical challenges.
Understanding the relationship between transcription and translation is crucial for cellular function.

Purpose of the Study:

To develop and validate a novel platform for deep, joint quantitative analysis of single-cell transcriptome and proteome.
To investigate transcript-protein correlations and the meiosis regulatory network in single mouse oocytes.

Main Methods:

Development of a single-cell simultaneous transcriptome and proteome (scSTAP) analysis platform.
Integration of microfluidics, high-throughput sequencing, and mass spectrometry.
Application to analyze single mouse oocytes during meiotic maturation.

Main Results:

Achieved average quantification of 19,948 genes and 2,663 protein groups per single mouse oocyte.
Enabled in-depth analysis of individual RNA-protein pair correlations and the meiosis regulatory network.
Identified 30 specific transcript-protein pairs as oocyte maturational signatures.

Conclusions:

The scSTAP platform provides a powerful resource for joint transcriptome and proteome analysis at the single-cell level.
The findings offer novel insights into transcriptional and translational regulation during oocyte meiosis.
Identified signatures can advance the study of cellular differentiation and regulatory mechanisms.