Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

In Situ Translation of Terminal Glycans into Covalent Readouts in Complex Biological Systems.

Analytical chemistry·2026

Same author

Electron-Ion Coupling for Nanostructured Photocathode in Ion-Assisted Photoelectrochemical Microfluidic Biosensing Platform.

Analytical chemistry·2026

Same author

Decoding the Spatiotemporal Logic of Cellular Senescence through Multimodal Exosomal miRNA Integration.

JACS Au·2026

Same author

Identification and quantification of intracellular N-acetylglucosamine modified glycoRNAs.

Nature communications·2026

Same author

Orthogonal recognition of miRNA and lncRNA enables high-fidelity cancer diagnostics.

Communications chemistry·2026

Same author

Synthetic Antibody Mimetics with ROS-Gated Saccharide Release for Targeted Colitis Therapy.

Advanced healthcare materials·2026

Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026

Same journal

Mitochondrial Redox Cascade-Directed Covalent NIR Fluorogenic Imaging of Therapy-Induced Senescence Integrates Tumor and Host Responses.

Analytical chemistry·2026

Same journal

Proteomic Profiling of RHD-Related Mitral Annulus Calcification Enabled by Magnetic Carbon Nanomaterial-Supported Quasi-Immobilized Enzyme Digestion.

Analytical chemistry·2026

Same journal

Spatial-Photonic Encoding on a Single Fiber: Breaking the Bottleneck in Photoelectrochemical Biosensing for Precision Diagnostics.

Analytical chemistry·2026

Same journal

Spreadable Biosensing Pregel for Analyte Visualization in Peeled Plant Tissues.

Analytical chemistry·2026

Same journal

DARibo-Q: RNA Allosteric Transduction for Fluorescence Imaging of Dopamine Modulation in Living Systems.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 11, 2025

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

Published on: May 27, 2012

Interception Proximity Labeling for Interrogating Cell Efflux Microenvironment.

Guyu Wang¹, Qiang Li¹, Yuna Guo¹

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Analytical Chemistry

|November 17, 2023

Summary

This summary is machine-generated.

Researchers developed an interception proximity labeling (IPL) platform to track extracellular hydrogen peroxide (H2O2) efflux. This tool visualizes oxidative stress and identifies proteins involved in cellular interactions, advancing redox signaling research.

More Related Videos

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Related Experiment Videos

Last Updated: Jul 11, 2025

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

Published on: May 27, 2012

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Published on: August 18, 2018

Area of Science:

Cell Biology
Biochemistry
Redox Biology

Background:

Extracellular hydrogen peroxide (H2O2) efflux is crucial for redox signaling but difficult to study.
A gap exists in understanding the extracellular link in redox signaling mechanisms.

Purpose of the Study:

To develop a novel platform for visualizing and analyzing extracellular H2O2 efflux.
To investigate oxidative stress in tumor cells and macrophages.
To identify proteins involved in macrophage-zymosan interactions.

Main Methods:

Developed an interception proximity labeling (IPL) platform by attaching horseradish peroxidase (HRP) to cell surface glycans.
Utilized endogenous H2O2 release as a trigger for proximity labeling.
Combined IPL with proteomics to identify interacting proteins.

Main Results:

Successfully visualized oxidative stress in nutrient-deprived tumor cells and stimulated macrophages.
Identified candidate proteins at the macrophage-zymosan interface using H2O2 interception labeling.
Confirmed glycan-dependent binding of zymosan to Toll-like receptor 2.

Conclusions:

The IPL platform provides a new method to study extracellular H2O2 dynamics.
This approach can elucidate mechanisms in biological processes involving redox pathways.
The study highlights the role of H2O2 in cellular responses and immune interactions.