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Related Experiment Video

Updated: Jul 11, 2025

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform

Published on: October 20, 2020

Improving DNA vaccination performance through a new microbubble design and an optimized sonoporation protocol.

Yuanchao Shi¹, Weixiong Weng¹, Mengting Chen¹

¹School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, Guangdong, China; National-regional Key Technology Engineering Laboratory for Medical Ultrasound, Shenzhen University, Shenzhen 518055, Guangdong, China.

Ultrasonics Sonochemistry

|November 17, 2023

Summary

Sonoporation, using ultrasound and microbubbles, enhances DNA vaccination by improving gene delivery and immune response. This novel method shows potential for safe and effective vaccine development.

Keywords:

DNA-loading capacity Long-term expression Microbubble Sustained antibody level Ultrasound Whole genome resequencing

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Last Updated: Jul 11, 2025

Production of Near-Infrared Sensitive, Core-Shell Vaccine Delivery Platform

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Published on: October 20, 2020

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

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Published on: June 12, 2011

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Published on: August 7, 2014

Area of Science:

Biotechnology
Immunology
Nanomedicine

Background:

Non-viral gene delivery methods are crucial for safe and effective vaccination.
Sonoporation, utilizing ultrasound and microbubbles, offers targeted gene delivery with immunostimulatory effects.

Purpose of the Study:

To investigate the application of sonoporation for enhancing DNA vaccination performance.
To develop an improved microbubble system for increased DNA loading and transfection efficiency.

Main Methods:

Development of cationic microbubbles loaded with DNA/PEI complexes.
In vitro and in vivo transfection studies using luciferase reporter gene.
Optimization of sonoporation protocols and immune cell activation analysis.
Assessment of antibody levels and gene expression duration using a hepatitis B DNA vaccine.

Main Results:

Novel microbubbles achieved high DNA loading capacity (0.25 pg/microbubble) and enhanced in vitro transfection.
Sonoporation increased in vivo transfection efficiency by up to 43.6-fold compared to intramuscular injection.
Sonoporation activated MHC-II+ immune cells and induced sustained high antibody levels for 56 weeks.
Achieved a record 400-day gene expression period with extrachromosomal gene state, confirming safety.

Conclusions:

Sonoporation is a highly effective non-viral method for DNA vaccination.
The developed microbubble system and optimized protocol significantly improve gene delivery and immune responses.
Sonoporation demonstrates potential for developing safe and efficient DNA vaccines with long-lasting effects.