Nanozyme catalyzed-SERRS sensor for the recognition of dopamine based on AgNPs@PVP with oxidase-like activity

Dopamine (DA), as one of the most significant neurotransmitters, is closely related to several diseases. Achieving rapid and sensitive detection of DA remains a challenge. Herein, we proposed a simple, fast, and sensitive method for DA recognition based on surface-enhanced resonance Raman scattering (SERRS) technique. The synthesized silver nanoparticles coated with polyvinylpyrrolidone (AgNPs@PVP) with oxidase activity could not only oxidize 3,3',5,5'-tetramethylbenzidine (TMB) directly to produce a blue oxidation state TMB (oxTMB) but also could be used as the SERS substrate to generate a strong SERRS signal. When DA was added to the above system, the blue color faded along with the decrease in the SERRS signal. The change value of SERRS intensity was in proportion to the concentration of DA in the range of 0.1-10 μM with a limit of detection of 40 nM. This method presented great potential for the recognition of DA-related diseases.