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Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

Mary Jasmine D Lara1, Sissy E Wamaitha2, Auriana Arabpour2

  • 1Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA.

Stem Cell Research
|November 17, 2023
PubMed

Insights

Researchers created a new rhesus macaque induced pluripotent stem cell (iPSC) line, riPSC05, from fetal fibroblasts. This cell line demonstrates pluripotency and self-renewal, validated through multiple characterization methods.

Area of Science:

  • Stem Cell Biology
  • Primate Research
  • Reproductive Biology

Background:

  • Induced pluripotent stem cells (iPSCs) are crucial for disease modeling and regenerative medicine.
  • Establishing well-characterized iPSC lines from non-human primates like rhesus macaques is vital for translational research.

Purpose of the Study:

  • To generate and characterize a novel induced pluripotent stem cell (iPSC) line from a rhesus macaque fetus.
  • To validate the pluripotency and genetic stability of the newly derived iPSC line for research applications.

Main Methods:

  • Fibroblasts were isolated from a rhesus macaque fetus.
  • Reprogramming was achieved using non-integrating Sendai virus technology.
  • Characterization involved assessing pluripotent markers, in vitro differentiation, karyotyping, and STR analysis.

Main Results:

  • A new rhesus macaque iPSC line, designated riPSC05, was successfully generated.
  • riPSC05 expressed key markers of pluripotency and self-renewal.
  • In vitro differentiation confirmed the potential to form all three germ layers.
  • Karyotyping and STR analysis confirmed genetic stability and authenticity.

Conclusions:

  • The riPSC05 line represents a valuable new resource for stem cell research in rhesus macaques.
  • This validated iPSC line can facilitate studies in developmental biology, disease modeling, and comparative genomics.

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