Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

8.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.5K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K
Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

6.4K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
6.4K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and Mechanistic Association Between Intestinal Permeability and the Gut Microbiome in Cirrhosis: Role of Phascolarctobacterium.

United European gastroenterology journal·2026
Same author

Presenilin-1 controls glycolysis and identity of pancreatic beta cells.

Communications biology·2026
Same author

A histidine switch controls the pH-responsive self-assembly of a helical protein filament.

bioRxiv : the preprint server for biology·2026
Same author

Cardiovascular effects of metyrapone treatment in patients with mild autonomous cortisol secretion: a secondary analysis.

The Journal of clinical endocrinology and metabolism·2026
Same author

Homocysteine and S-adenosyl-L-homocysteine impair development and methylation in yeast and flies.

Disease models & mechanisms·2026
Same author

MyBioScope: a new frontier in gut microbiome and health research.

Bioresources and bioprocessing·2026

Related Experiment Video

Updated: Jul 11, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.2K

Targeted perturbation of signaling-driven condensates.

Tianshu Gui1, Cassio Fleming2, Caterina Manzato2

  • 1Center for Molecular Medicine, University Medical Center Utrecht, 3584 CG Utrecht, the Netherlands; Oncode Institute, 3721 AL Utrecht, the Netherlands.

Molecular Cell
|November 17, 2023
PubMed
Summary

Scientists developed peptides to disrupt cancer-driving protein condensates. These peptides target specific cellular condensates, inhibiting oncogenic signaling and cancer cell growth, offering a new therapeutic strategy.

Keywords:
NMRWNT-signalingbiomolecular condensatessignaling inhibitiontranscriptional regulation

More Related Videos

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.7K
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.9K

Related Experiment Videos

Last Updated: Jul 11, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.2K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.7K
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.9K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Biomolecular condensates are crucial for cellular organization but their dynamics are poorly understood.
  • Transcriptional machinery forms condensates at key genes to regulate cell identity.
  • Dysregulation of these condensates contributes to oncogenic signaling.

Purpose of the Study:

  • To investigate the disruption of WNT-activated β-catenin condensates in human cancer cells.
  • To identify specific molecular inhibitors of condensate formation and function.
  • To explore therapeutic strategies targeting condensate-associated transcription in cancer.

Main Methods:

  • Live-cell condensate imaging in human cancer cells.
  • Discovery and application of FOXO and TCF-derived peptides.
  • Analysis of peptide competition with homotypic intermolecular interactions.
  • Investigation of Hippo pathway components YAP and TAZ.

Main Results:

  • Identified peptides that specifically inhibit β-catenin condensate formation on DNA.
  • Demonstrated perturbation of nuclear β-catenin condensates and downstream transcriptional activation.
  • Showed inhibition of colorectal cancer cell growth.
  • Derived peptides that perturb YAP/TAZ condensates and transcription in the Hippo pathway.

Conclusions:

  • FOXO and TCF-derived peptides effectively inhibit oncogenic signaling by disrupting β-catenin condensates.
  • A 'monomer saturation' model explains how short peptides can inhibit condensate-associated transcription.
  • This approach offers a novel strategy for targeting aberrant transcription in diseases like cancer.