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Probing behavior of the leafhopper analyzed through DC electropenetrography and microscopy
Jariya Roddee1, Jureemart Wangkeeree2, Elaine A Backus3
1School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Suranaree, Muang, 30000, Nakhon Ratchasima, Thailand.
Journal of Insect Physiology
|November 17, 2023
Summary
Yamatotettix flavovittatus leafhoppers primarily feed on sugarcane phloem sap. Adults exhibit more frequent and prolonged feeding than nymphs, suggesting a greater role in transmitting sugarcane white leaf phytoplasma.
Area of Science:
- Entomology
- Plant Pathology
- Agricultural Science
Background:
- Yamatotettix flavovittatus is a leafhopper vector of sugarcane white leaf (SCWL) phytoplasma.
- SCWL phytoplasma causes significant sugarcane chlorosis, impacting crop yield.
- The probing behavior of Y. flavovittatus and its role in SCWL phytoplasma transmission require detailed investigation.
Purpose of the Study:
- To characterize the probing behaviors of adult and nymphal Y. flavovittatus on sugarcane using electropenetrography (EPG).
- To correlate EPG waveforms with specific feeding activities and stylet activities within sugarcane tissues.
- To elucidate the feeding preferences and transmission potential of Y. flavovittatus for SCWL phytoplasma.
Main Methods:
- DC electropenetrography (EPG) was employed to record electricalPenetration_Graphs (EPGs) of adult and fifth-instar nymphal Y. flavovittatus on sugarcane.
- Salivary sheath termini were analyzed using light and scanning electron microscopy to determine stylet tip locations.
- Six distinct EPG waveforms (NP, Yf1-Yf5) were identified and associated with specific probing and feeding activities.
Main Results:
- Y. flavovittatus predominantly feeds on phloem sieve elements, ingesting sap more frequently and for longer durations than other cell types.
- Adult leafhoppers exhibited longer total probing durations and higher densities of puncture holes compared to nymphs.
- Adults demonstrated more frequent and prolonged phloem sap ingestion than nymphs, indicating enhanced feeding efficiency.
Conclusions:
- Y. flavovittatus is confirmed as a primary phloem feeder on sugarcane.
- Adult Y. flavovittatus possess a greater capacity for acquiring and inoculating SCWL phytoplasma due to enhanced feeding behaviors.
- Understanding leafhopper penetration behavior is crucial for developing strategies to manage SCWL phytoplasma transmission.
