Deciphering survival strategies: Oxidative stress and microbial interplay in Eisenia fetida under tetracycline...

Soil contamination resulting from residual antibiotics presents a pressing need to understand the survival mechanisms of soil organisms in polluted environments. This study focused on Eisenia fetida, and tetracycline stress experiments were conducted in a controlled environment using sterile artificial soil. The stress concentration ranged from 0 to 600 mg/kg, and stress cycles lasted either 10 or 30 days. The objective of this study was to assess the effects of oxidative stress and the changes in microbial communities both within and outside the earthworms. A comprehensive mathematical model was developed to elucidate the responses of organisms following exposure to stress utilizing factor analysis, grey relational analysis, and hierarchical entropy weight analysis. Under tetracycline stress, the initial stage (1-3 days) exhibited a coordinated regulation of oxidative stress and microbial communities in the soil with the assistance of CAT and GPX enzymes. The subsequent stage (4-5 days) further emphasized the influence of soil microbial communities. A notable "feedback regulation" of soil microbial communities on oxidative stress was observed during the third stage (6-8 days). Earthworms maintained a metabolic balance in the fourth stage (9-10 days). In the long term, the stress-induced a self-detoxification mechanism within soil microbial communities, which collaborated with GPX to respond to oxidative stress.