Fate and disposition of [¹⁴C]-polystyrene microplastic after oral administration to laying hens

Micro/nanoplastics (MP) are emerging environmental contaminants of great concern because of their ubiquitous distribution in air, soil, water, and food. Reports have described MP in the excreta of food animals, but their absorption, distribution, and elimination in terrestrial animals used for human consumption is essentially unexplored. To determine the absorption and distribution of [¹⁴C]-polystyrene (PS) MP, laying hens (n = 15) were bolus dosed with 10 μCi/hen (11.1 ± 0.8 mg/kg) and the extent of radioactivity in blood and tissues was determined in birds harvested on withdrawal days (WD) 1, 2, 3, 4, and 7 (3 hens per WD). Radiocarbon was also determined in egg fractions and excreta collected throughout the study. Blood, eggs, and tissues contained a total of <1 % of the administered dose, demonstrating that polystyrene microparticles were poorly absorbed. Recovery of radioactivity in excreta within the first withdrawal day was nearly quantitative (96.8 ± 14.5 %, n = 15), suggesting exposure of poultry to dietary PS-MP would not likely represent subsequent food safety risks and that most PS-MP present in poultry diets would return to the environment.