Related Experiment Video
Updated: Jul 8, 2026

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
Published on: June 1, 2014
The colors of peroxygenase activity: Colorimetric high-throughput screening assays for directed evolution
Mikel Dolz1, Dianelis T Monterrey1, Alejandro Beltrán-Nogal1
1Department of Biocatalysis, Institute of Catalysis, CSIC, C/ Marie Curie 2, Cantoblanco, Madrid, Spain.
Abstract:
Fungal unspecific peroxygenases (UPOs) are arising as versatile biocatalysts for C-H oxyfunctionalization reactions. In recent years, several directed evolution studies have been conducted to design improved UPO variants. An essential part of this protein engineering strategy is the design of reliable colorimetric high-throughput screening (HTS) assays for mutant library exploration. Here, we present a palette of 12 colorimetric HTS assays along with their step-by-step protocols, which have been validated for directed UPO evolution campaigns. This array of colorimetric assays will pave the way for the discovery and design of new UPO variants.
Related Concept Videos
Redox Titration: Overview
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
Evolution of New Traits in Microbes

