Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 10, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.2K

Modeling phenotypic heterogeneity towards evolutionarily inspired osteosarcoma therapy.

Darcy L Welch1,2, Brooke L Fridley3, Ling Cen3

  • 1Adolescent and Young Adult Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.

Scientific Reports
|November 18, 2023
PubMed

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Paediatric Therapeutic Development Workshop on rhabdomyosarcoma.

British journal of cancer·2026
Same author

Adaptive and sequential cancer therapies emerge from treatment schedule optimization.

Research square·2026
Same author

Integrated Genomic and Epigenomic Analysis Reveals Epigenetic Plasticity in Disease Progression and Multidrug Resistance in Multiple Myeloma.

Cancer research·2026
Same author

Which evolutionary game-theoretic model best captures NSCLC dynamics?

PloS one·2026
Same author

Pembrolizumab plus high-dose IL-2 in advanced clear cell renal cell carcinoma: six-year survival outcomes and molecular signatures from a phase 2 trial.

Nature communications·2026
Same author

Spatial Clustering of Recently Activated Cytotoxic Lymphocytes Improves Association with Overall Survival in Women with High-Grade Serous Ovarian Cancer.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
Same journal

Efficacy of historical context and exogenous features on deep learning for cooling load forecasting in chilled water plants.

Scientific reports·2026
See all related articles
Summary
This summary is machine-generated.

Intratumoral heterogeneity in osteosarcoma (bone cancer) impacts chemotherapy effectiveness. This study models heterogeneity to reveal how environmental changes and sequential therapies can overcome treatment resistance in patients.

Area of Science:

  • Oncology
  • Cancer Biology
  • Biotechnology

Background:

  • Osteosarcoma is the most common bone cancer in pediatric and young adult populations.
  • Chemotherapy resistance, often driven by intratumoral heterogeneity, limits treatment efficacy in approximately half of localized osteosarcoma cases.
  • Understanding cellular heterogeneity is crucial for developing more effective therapeutic strategies.

Purpose of the Study:

  • To develop and characterize an in vitro model of osteosarcoma heterogeneity based on phenotypic and genotypic variations.
  • To investigate how environmental and chemical perturbations influence the behavior of co-cultured osteosarcoma cell lines.
  • To evaluate therapeutic strategies for overcoming chemoresistance in heterogeneous osteosarcoma models.

Main Methods:

More Related Videos

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study
02:35

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study

Published on: March 22, 2024

833
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

9.3K

Related Experiment Videos

Last Updated: Jul 10, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.2K
Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study
02:35

Author Spotlight: Replicating Human Osteosarcoma Progression in Immunodeficient Mice for Cancer Study

Published on: March 22, 2024

833
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

9.3K
  • Genotypic and phenotypic characterization of an osteosarcoma cell line panel, focusing on co-cultures of divergent lines (143B and SAOS2).
  • Assessment of cell line competition under varying environmental conditions (e.g., pH, glutamine levels) and chemotherapy exposure.
  • Evaluation of spheroid formation and response to single-agent and sequential chemotherapy treatments in a two-cell line co-culture model.

    • Main Results:

    • Environmental factors (pH, glutamine) and chemotherapy significantly altered the composition and success of co-cultured osteosarcoma cell lines.
    • Under nutrient-rich conditions, 143B cells outcompeted SAOS2 cells, but SAOS2 dominance was observed in nutrient-deprived or chemotherapy-treated spheroids.
    • Sequential chemotherapy, switching from a first-strike to a second-strike agent, was the only evaluated approach effective in eliminating heterogeneous spheroids.

    Conclusions:

    • The developed in vitro model effectively recapitulates osteosarcoma heterogeneity and its impact on therapeutic response.
    • Environmental conditions and cell-cell interactions within heterogeneous populations critically influence treatment outcomes.
    • Sequential therapeutic strategies are essential for overcoming chemoresistance mediated by intratumoral heterogeneity in osteosarcoma.