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Updated: Jul 10, 2026

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals
Published on: October 20, 2019
Protocol for the integration of fiber photometry and social behavior in rodent models
Dylan J Terstege1, Matthew Dawson2, Naila F Jamani2
1Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 1N4, Canada; Hotchkiss Brain Institute, University of Calgary, Calgary, AB T2N 1N4, Canada.
Abstract:
Fiber photometry offers insight into cell-type-specific activity underlying social interactions. We provide a protocol for the integration of fiber photometry recordings into the analysis of social behavior in rodent models. This includes considerations during surgery, notes on synchronizing fiber photometry with behavioral recordings, advice on using multi-animal behavioral tracking software, and scripts for the analysis of fiber photometry recordings. For complete details on the use and execution of this protocol, please refer to Dawson et al. (2023).1.
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