Optimizing coupling layer and superstrate thickness in attachable acoustofluidic devices

Superstrate-based acoustofluidic devices, where the fluidic elements are reversibly coupled to a transducer rather than bonded to it, offer advantages for cost, interchangeability and preventing contamination between samples. A variety of coupling materials can be used to transmit acoustic energies into attachable superstrates, though the dimensions and material composition of the system elements are not typically optimized. This work analyzes these coupling layers for bulk wavefront transmission, including water, ultrasound gel and polydimethylsiloxane (PDMS), as well as the material makeup and thickness of the superstrate component, which is commonly comprised of glass, quartz or silicon. Our results highlight the importance of coupling layer and superstrate dimensions, identifying frequencies and component thicknesses that maximize transmission efficiency. Our results indicate that superstrate thicknesses 0.55 times the acoustic wavelength result in maximal acoustic coupling. While various coupling layers and superstrate materials are capable of similar acoustic energy transmission, the inherent dimensional stability of the PDMS coupling layers, somewhat less common in superstrate work compared to liquid-based agents, presents advantages for practically maximizing acoustic efficiency.