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Updated: Jul 10, 2025
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
34.0K
High-level RNA editing diversifies the coleoid cephalopod brain proteome
Gjendine Voss1, Joshua J C Rosenthal1
1The Eugene Bell Center, The Marine Biological Laboratory, 7 MBL Street, Woods Hole MA 02543, United States.
Briefings in Functional Genomics
|November 20, 2023
Summary
Cephalopods extensively recode proteins using adenosine deaminase acting on RNA (ADAR) editing. This RNA recoding is temperature-sensitive, suggesting an adaptive role, though mechanisms remain unclear.
Area of Science:
- Neurobiology
- Molecular Biology
- Genetics
Background:
- Coleoid cephalopods possess complex nervous systems.
- They uniquely recode most proteins via adenosine to inosine (A-to-I) RNA editing.
- This editing involves adenosine deaminase acting on RNA (ADAR) enzymes, altering protein sequences.
Purpose of the Study:
- Investigate mechanisms behind high-level RNA recoding in cephalopods.
- Explore the adaptive potential of this extensive RNA editing.
- Characterize ADAR enzymes and temperature sensitivity in cephalopods.
Main Methods:
- Analysis of ADAR enzyme complements in cephalopods.
- Review of existing literature on RNA editing functions and temperature sensitivity.
- Examination of recent genome editing advancements in squid.
Main Results:
- Identified ADAR enzyme complements, including a novel domain in sqADAR1.
- Summarized evidence for an adaptive role of RNA recoding.
- Highlighted temperature sensitivity in a significant proportion of recoding sites.
Conclusions:
- High-level RNA recoding in coleoids is a significant biological phenomenon.
- Temperature sensitivity suggests an adaptive function for RNA editing.
- Further research, potentially using squid genome editing, is needed to understand these mechanisms.
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