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circ_0006528 promotes nonsmall cell lung cancer progression by sponging miR-892a and regulating NRAS expression

Weixi Guo1, Hongming Liu1, Ming Zhong1

  • 1Department of Thoracic Surgery, The First Affiliated Hospital of Xiamen University.

Anti-Cancer Drugs
|November 20, 2023
PubMed

Insights

Circular RNA circ_0006528 promotes non-small cell lung cancer (NSCLC) progression by sponging miR-892a to increase NRAS expression. Inhibiting circ_0006528 suppresses NSCLC growth and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-small cell lung cancer (NSCLC) progression and drug resistance are influenced by micro-RNAs, but their mechanisms are not fully understood.
  • Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, including NSCLC.

Purpose of the Study:

  • To investigate the role of circ_0006528 in NSCLC development and drug resistance.
  • To elucidate the mechanism by which circ_0006528 regulates NSCLC cell progression via sponging miR-892a and affecting neuroblastoma rat sarcoma viral oncogene (NRAS) expression.

Main Methods:

  • Identification of circ_0006528 using PCR and RNase R treatment.
  • Assessment of cell proliferation, invasion, and migration using MTT, Transwell, and scratch assays in NSCLC cell lines (SPCA-1 and A549) after circ_0006528 knockdown.
  • Luciferase reporter assays to confirm the interaction between circ_0006528, miR-892a, and NRAS.
  • Functional recovery assays using miR-892a inhibitors.
  • In vivo studies using a xenograft mouse model.

Main Results:

  • circ_0006528 was found to be highly expressed in NSCLC tissues compared to non-cancerous tissues.
  • Knockdown of circ_0006528 significantly reduced NSCLC cell proliferation and viability.
  • circ_0006528 directly binds to miR-892a, which in turn targets NRAS.
  • Inhibition of miR-892a reversed the effects of circ_0006528 knockdown, restoring NSCLC cell proliferation, invasion, and migration.

Conclusions:

  • circ_0006528 acts as a crucial oncogene in NSCLC.
  • The circ_0006528/miR-892a/NRAS axis plays a significant role in regulating NSCLC cell growth and invasion.
  • Targeting circ_0006528 may represent a potential therapeutic strategy for NSCLC treatment.

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