Related Experiment Video
Updated: Jul 10, 2025
10:28
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
Published on: July 15, 2018
9.5K
Complete genome sequence of Bacillus subtilis subsp. natto NARUSE using PacBio sequencing
Yukie Kubo1,2, Yoshiyuki Tanizaki1,2, Daisuke Hira3
1Kyushu Soy Food Co., Ltd., Kumamoto, Japan.
Microbiology Resource Announcements
|November 20, 2023
Summary
We sequenced the full genome of Bacillus subtilis subsp. natto NARUSE, a bacterium used in traditional Japanese natto fermentation. This provides a foundational resource for understanding its unique fermentation capabilities and genetic makeup.
Area of Science:
- Microbiology
- Genomics
- Food Science
Background:
- Bacillus subtilis subsp. natto is crucial for fermenting soybeans into natto, a traditional Japanese food.
- Understanding the genetic makeup of this microorganism is essential for optimizing fermentation processes and ensuring food safety.
Purpose of the Study:
- To determine the complete genome sequence of Bacillus subtilis subsp. natto NARUSE.
- To provide a genomic resource for future research on Bacillus subtilis subsp. natto.
Main Methods:
- Whole-genome sequencing was performed using the PacBio sequencing system.
- Bioinformatic analysis was conducted to assemble and annotate the genome.
Main Results:
- The complete genome sequence of Bacillus subtilis subsp. natto NARUSE was obtained.
- The genome consists of a circular chromosome of 4,148,793 nucleotides and a plasmid of 62,770 nucleotides.
Conclusions:
- The availability of the complete genome sequence facilitates further studies into the genetic basis of natto fermentation.
- This genomic data can aid in strain improvement and the development of novel fermentation technologies.
Related Concept Videos
01:57Sanger Sequencing
754.6K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.6K
03:21RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K