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Microwave-assisted Functionalization of Polyethylene glycol and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
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Facile Physicochemical Reprogramming of PEG-Dithiolane Microgels.

Benjamin R Nelson1,2, Bruce E Kirkpatrick1,2,3, Nathaniel P Skillin1,2,3

  • 1Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO, 80303, USA.

Advanced Healthcare Materials
|November 20, 2023
PubMed
Summary
This summary is machine-generated.

Researchers transformed spherical microgels into disk shapes using dynamic disulfide crosslinks and compression. This shape change influences cell behavior and the self-assembly of granular biomaterials for tissue engineering.

Keywords:
PEG hydrogelscurvaturedithiolanesdynamic materialsmicrogels

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Granular biomaterials are crucial in tissue engineering due to porosity, tunable properties, and printability.
  • Current methods for complex microgel geometries are limited, often requiring microfluidics or in-mold polymerization.

Purpose of the Study:

  • To develop a novel method for creating anisotropic microgel shapes.
  • To investigate the impact of microgel shape on cell behavior and scaffold assembly.

Main Methods:

  • Fabrication of photopolymerized microgels using dithiolane-functionalized macromolecules via batch emulsion.
  • Transformation of spherical microgels into disks through unconfined compression with photoinitiated radicals.
  • Culturing C2C12 myoblasts on disk-shaped microgels and observing cell localization and supraparticle assembly.

Main Results:

  • Spherical microgels were successfully reshaped into disks, with flattened contact areas and increased boundary curvature.
  • Cells (C2C12 myoblasts) preferentially localized to regions of higher curvature on the disk surfaces.
  • The anisotropic shape of disk microgels directed their self-assembly onto curved surfaces, unlike spherical counterparts.

Conclusions:

  • A novel spatiotemporal process enables rapid reprocessing of microgels into anisotropic shapes.
  • Microgel shape significantly influences cell localization and subsequent cell-driven assembly of supraparticle scaffolds.
  • This work opens new avenues for studying shape-driven mechanobiological cues in granular hydrogel assembly.