Related Experiment Video
Updated: Jul 10, 2025

The Use of Induced Somatic Sector Analysis ISSA for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
Published on: October 5, 2016
Transcriptomic monitoring of Douglas-fir heartwood formation
Didier Delourme1, Laure Brémaud1, Idelette Plazanet1
1Université de Limoges, LABCIS, UR 22722, 123 avenue Albert Thomas, Limoges, F-87060, France.
Objectives:
Molecular cues linked to heartwood formation open new (complementary) perspectives to genetic breeding programs of Douglas-fir, a tree species largely cultivated in Europe for the natural durability and civil engineering properties of its wood.
Data Description:
RNAs from a single genotype of Douglas-fir, extracted from three distinct wood zones (outer sapwood, inner sapwood and transition zone) at four vegetative seasons to generate an extensive RNA-seq dataset used to apprehend the in-wood dynamic and seasonality of heartwood formation in this hardwood model species. Previously published data collected on somatic embryos of the same genotype could be merged with the present dataset to upgrade grade the Douglas-fir reference transcriptome.

