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Upregulated TC1 and downregulated Chibby were correlated with the aberrant β-catenin expression in laryngeal squamous

Gang Ren1, Bingliang Ma1, Jianqiu Wang1

  • 1Department of Otolaryngology, the First People's Hospital of Huzhou, the First affiliated Hospital of Huzhou university, Huzhou, Zhejiang, China.

Medicine
|November 21, 2023
PubMed

Insights

Thyroid cancer 1 (TC1) upregulation and Chibby downregulation correlate with aberrant beta-catenin expression in laryngeal squamous cell carcinoma (LSCC). These changes are linked to increased Cyclin D1 and MMP-7, but not overall survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Aberrant beta-catenin expression is implicated in various cancers, playing a key role in the Wnt/beta-catenin signaling pathway.
  • Chibby acts as an antagonist in this pathway, while thyroid cancer 1 (TC1) is an activator that can relieve Chibby's inhibitory effects.
  • TC1 is frequently overexpressed in human tumors, suggesting its potential role in cancer development.

Purpose of the Study:

  • To investigate the expression levels of TC1, Chibby, and beta-catenin in laryngeal squamous cell carcinoma (LSCC).
  • To explore the associations among TC1, Chibby, and beta-catenin expression in LSCC.
  • To examine the correlation of these proteins with clinicopathological features and downstream targets like c-Myc, Cyclin D1, and MMP-7 in LSCC.

Main Methods:

  • Immunohistochemistry was employed to assess the expression of TC1, Chibby, beta-catenin, c-Myc, Cyclin D1, and MMP-7 in 53 LSCC patient samples.
  • Expression levels were compared between LSCC tissues and normal laryngeal squamous epithelium (NLSE).
  • Statistical analyses were performed to correlate protein expression with clinicopathological parameters and patient survival.

Main Results:

  • LSCC tissues exhibited upregulated TC1, downregulated Chibby, and aberrant cytoplasmic beta-catenin compared to NLSE (P < .001).
  • High TC1 expression correlated with advanced tumor site, TNM and T stage, lymphovascular invasion, and poor differentiation (P < .050).
  • Aberrant beta-catenin expression was associated with tumor site, advanced TNM and T stage, lymphovascular invasion, perineurial invasion, and poor differentiation (P < .050).
  • Upregulated TC1 and downregulated Chibby were significantly correlated with aberrant beta-catenin expression (P < .001).
  • High expression of Cyclin D1 and MMP-7 was observed in LSCC tissues and correlated with TC1/Chibby expression patterns (P < .001).
  • No significant association was found between the expression of TC1, Chibby, beta-catenin, and overall patient survival (P > .05).

Conclusions:

  • TC1 upregulation and Chibby downregulation are associated with aberrant beta-catenin signaling in LSCC.
  • These molecular changes correlate with aggressive clinicopathological features and elevated expression of Cyclin D1 and MMP-7.
  • The study suggests a role for the TC1/Chibby/beta-catenin axis in LSCC progression, independent of overall survival.

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