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Correction: Islam, R.A.; Rallis, C. Ribosomal Biogenesis and Heterogeneity in Development, Disease, and Aging.
Rowshan Ara Islam1,2, Charalampos Rallis1,2
1School of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.
Epigenomes
|November 21, 2023
Summary
Errors in mammalian 5' untranslated region (UTR) annotations can lead to the identification of false-positive internal ribosome entry sites (IRESes). This study highlights the need for accurate gene annotation in molecular biology research.
Area of Science:
- Molecular Biology
- Genetics
- Bioinformatics
Context:
- Accurate gene annotation is crucial for understanding gene expression and regulation.
- Internal ribosome entry sites (IRESes) mediate cap-independent translation initiation.
- Previous studies have relied on existing annotations for IRES identification.
Purpose:
- To investigate the impact of mammalian 5' UTR annotation errors on IRES identification.
- To identify specific genes where annotation errors lead to false-positive IRES findings.
- To provide a corrected analysis of IRES elements in affected genes.
Summary:
- Errors in mammalian 5' UTR annotations were found to cause false-positive identification of internal ribosome entry sites (IRESes).
- Specific genes, including Hoxa9, were identified as exhibiting these annotation-driven false positives.
- The study emphasizes the critical need for precise 5' UTR annotation in the accurate discovery of functional IRES elements.
Impact:
- This research improves the reliability of IRES element identification by addressing annotation-related artifacts.
- It provides a foundation for re-evaluating IRES function in genes with previously misannotated 5' UTRs.
- Enhanced accuracy in gene annotation will benefit future studies on translation regulation and gene expression.