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Updated: Jul 21, 2026

Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

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MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in

Pedro J Almiñana-Pastor1, Francisco M Alpiste-Illueca1, Pablo Micó-Martinez1

  • 1Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, 46010 Valencia, Spain.

Non-Coding RNA
|November 21, 2023
PubMed
Abstract

Insights

Gingival crevicular fluid microRNAs (miRNAs) show promise as periodontal disease biomarkers. Sequencing identified miR-199 as a key indicator for chronic periodontitis, offering potential diagnostic applications.

Area of Science:

  • Biomolecular research
  • Oral health diagnostics
  • Genomics

Background:

  • MicroRNAs (miRNAs) in gingival crevicular fluid (GCF) are potential biomarkers for periodontal disease.
  • Chronic periodontitis is a prevalent oral condition requiring accurate diagnostic tools.

Purpose of the Study:

  • To conduct a comprehensive miRNA-sequencing of GCF.
  • To compare miRNA expression profiles between chronic periodontitis (CP) patients and healthy subjects (HS).

Main Methods:

  • GCF samples collected from severe CP patients (n=11) and HS (n=12).
  • miRNAs isolated using an miRNeasy Serum/Plasma kit.
  • Expression levels determined by reverse transcription quantitative polymerase chain reaction (qRT-PCR).

Main Results:

  • Sequencing identified miR-199, miR-146a, miR-30a, and miR-338 in CP patient GCF.
  • miR-199 demonstrated the highest potential as a biomarker for periodontitis.

Conclusions:

  • This study is the first to sequence all known miRNAs in GCF.
  • Identified potential GCF-derived miRNA biomarkers for periodontitis.
  • High-throughput sequencing clarifies the role of miRNAs in periodontitis and their diagnostic potential.
Keywords:
epigeneticsgingival crevicular fluidmicroRNAsperiodontitis

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