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Published on: October 20, 2023
Molecular Organization of the Skin Barrier
1Department of Cellular and Molecular Biology (CMB), Karolinska Institutet, Biomedicum, SE-171 77 Stockholm, Sweden. Lars.Norlen@ki.se.
Abstract:
Cryo-electron microscopy of vitreous sections allows for investigation directly in situ of the molecular architecture of skin. Recently, this technique has contributed to the elucidation of the molecular organization of the skin's permeability barrier and its stepwise formation process. The aim of this review is to provide an overview of the procedure for cryo-electron microscopy of vitreous sections, its analysis using atomic detail molecular dynamics modelling and electron microscopy simulation, and its application in the investigation of the barrier structure and formation process of the skin.
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