Related Experiment Video
Updated: Jul 10, 2025
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
Published on: February 27, 2021
Begomovirus Inoculation in Arabidopsis and Cassava
José T Ascencio-Ibáñez1, Mary M Dallas2, Linda Hanley-Bowdoin2
1Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, USA. jtascenc@ncsu.edu.
Abstract:
The use of infectious clones to inoculate plant viruses allows for controlled studies that lead to a better understanding of plant-virus interactions. The main methods used for laboratory inoculation of geminiviruses are agroinoculation and biolistics. We describe how to successfully inoculate geminiviruses, focusing on Arabidopsis as a model plant and cassava as a crop.
