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Updated: Jul 10, 2025

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
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NADPH oxidase 4-mediating oxidative stress contributes to endometriosis

Xiaojie Wang1, Xiaona Jiang1, Xin Lv1

  • 1Department of Gynecology, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, No.20 Yuhuangding East Road, Yantai, 264000, Shandong, China.

Journal of Applied Genetics
|November 22, 2023
PubMed

Insights

NADPH oxidase 4 (NOX4) is elevated in endometriosis and linked to oxidative stress. Targeting NOX4 may offer a therapeutic strategy by regulating oxidative stress markers in endometrial cells.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Endometriosis is a condition linked to oxidative stress.
  • NADPH oxidase 4 (NOX4) is implicated in regulating oxidative stress.

Purpose of the Study:

  • To investigate the role of NOX4 in endometriosis.
  • To elucidate the molecular mechanisms of NOX4 in this condition.

Main Methods:

  • Collected clinical specimens and isolated human endometrial stromal cells (HESCs).
  • Established NOX4 knockdown HESC lines and induced them with peritoneal fluid.
  • Quantified NOX4 and oxidative stress markers using IHC, western blotting, qPCR, and ELISAs.
  • Analyzed correlations using Pearson correlation coefficient.

Main Results:

  • NOX4 levels were significantly higher in ectopic endometrium compared to normal endometrium.
  • Oxidative stress biomarkers were dysregulated in ectopic endometrium.
  • A positive correlation was found between NOX4 and oxidative stress biomarkers.
  • NOX4 modulated oxidative stress markers in HESCs stimulated by endometriosis peritoneal fluid.

Conclusions:

  • NOX4 is upregulated in endometriosis and associated with oxidative stress.
  • NOX4 plays a role in endometriosis pathogenesis, partly through regulating oxidative stress.
Keywords:
CatalaseEndometrial stromal cellsEndometriosisNADPH oxidase 4Oxidative stress

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