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Updated: Jul 10, 2025

Quantifying Fitness Costs in Transgenic Aedes aegypti Mosquitoes
Published on: September 15, 2023
AePUb promoter length modulates gene expression in Aedes aegypti
Michelle A E Anderson1,2, Philip T Leftwich3,4, Ray Wilson3,5
1Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK. michelle.anderson@york.ac.uk.
Abstract:
Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
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