Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

5.8K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K
Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.4K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.4K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

4.8K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Climatic reconstruction at the Miocene Shanwang basin, China, using leaf margin analysis, CLAMP, coexistence approach, and overlapping distribution analysis.

American journal of botany·2011
Same author

Novel candidate colorectal cancer biomarkers identified by methylation microarray-based scanning.

Endocrine-related cancer·2011
Same author

Stress and strain analysis of contractions during ramp distension in partially obstructed guinea pig jejunal segments.

Journal of biomechanics·2011
Same author

Role of Gα(12)- and Gα(13)-protein subunit linkage of D(3) dopamine receptors in the natriuretic effect of D(3) dopamine receptor in kidney.

Hypertension research : official journal of the Japanese Society of Hypertension·2011
Same author

Transarticular screw and C1 hook fixation for os odontoideum with atlantoaxial dislocation.

World neurosurgery·2011
Same author

Surgical treatments of myelopathy caused by cervical ligamentum flavum ossification.

World neurosurgery·2011
Same journal

Cardiac xenotransplantation: Progress, barriers, and pathways toward clinical translation.

Journal of biomedical research·2026
Same journal

Allogeneic CAR-T cell therapies: Overcoming clinical challenges and therapeutic potential against tumors.

Journal of biomedical research·2026
Same journal

Surface electromyography for evaluating the impact of focused ultrasound on the sciatic nerve modulation in rats.

Journal of biomedical research·2026
Same journal

Paeoniflorin elevates the expression of SOCS3 in macrophages to prevent MIA-induced osteoarthritis in mice.

Journal of biomedical research·2026
Same journal

ETS1 activates heparanase transcription to drive intra-islet heparan sulfate degradation and macrophage-induced insulitis in type 1 diabetes.

Journal of biomedical research·2026
Same journal

Editorial commentary on the special section on cancer research.

Journal of biomedical research·2026
See all related articles

Related Experiment Video

Updated: Jul 10, 2025

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
09:19

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

8.7K

Cisplatin increases carboxylesterases through increasing PXR mediated by the decrease of DEC1.

Minqin Xu1, Lihua Zhang1, Lan Lin1

  • 1Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Journal of Biomedical Research
|November 22, 2023
PubMed
Summary
This summary is machine-generated.

cis-Diamminedichloroplatinum (CDDP) increases carboxylesterase 1 and 2 (CES1/CES2) by upregulating the pregnane X receptor (PXR) via decreased differentiated embryonic chondrocyte-expressed gene 1 (DEC1). This mechanism enhances cancer treatment potential.

Keywords:
carboxylesterase 1carboxylesterase 2cis-diamminedichloroplatinumdifferentiated embryonic chondrocyte-expressed gene 1irinotecanpregnane X receptor

More Related Videos

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

960
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

4.9K

Related Experiment Videos

Last Updated: Jul 10, 2025

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
09:19

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Published on: February 6, 2015

8.7K
Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

960
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

4.9K

Area of Science:

  • Pharmacology
  • Molecular Biology
  • Hepatology

Background:

  • cis-Diamminedichloroplatinum (CDDP) is a cornerstone chemotherapy for solid tumors.
  • Understanding CDDP's molecular mechanisms is crucial for optimizing cancer therapy.

Purpose of the Study:

  • To elucidate the molecular pathways through which CDDP influences carboxylesterase expression.
  • To investigate the roles of pregnane X receptor (PXR) and differentiated embryonic chondrocyte-expressed gene 1 (DEC1) in CDDP's effects.

Main Methods:

  • Assessed CDDP's impact on CES1/CES2 expression and activity in human hepatoma cells and mouse liver/intestine.
  • Utilized gene overexpression and knockdown techniques for PXR and DEC1.
  • Performed reporter assays to evaluate transcriptional regulation of DEC1.

Main Results:

  • CDDP significantly increased CES1 and CES2 expression and activity.
  • PXR activation was identified as a key mediator of CDDP-induced carboxylesterase upregulation.
  • DEC1 downregulation was found to be upstream of PXR activation, suggesting a novel regulatory axis.
  • CDDP suppressed DEC1 transcriptionally.
  • Combined CDDP and irinotecan showed synergistic effects, particularly when CDDP was administered first.

Conclusions:

  • CDDP enhances carboxylesterase expression through a pathway involving PXR activation and DEC1 downregulation.
  • This study reveals a novel mechanism for CDDP action with implications for combination therapies.
  • Targeting the DEC1-PXR-CES axis may offer new strategies in cancer treatment.