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Genome sequence of Microbacterium foliorum bacteriophage DumpQuist isolated from soil in Clarksville, Tennessee

Sergei A Markov1, Kehinde O Olusoga1, Patience O Oni1

  • 1Biology Department, Austin Peay State University, Clarksville, Tennessee, USA.

Microbiology Resource Announcements
|November 22, 2023
PubMed

Insights

Researchers isolated the DumpQuist bacteriophage from Tennessee soil, identifying its podovirus structure and genome. This phage is closely related to others in the actinobacteriophage EK1 cluster.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
  • Actinobacteriophages are bacteriophages that specifically infect Actinobacteria, a diverse group of bacteria.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage from soil samples.
  • To determine the morphology and genomic features of the isolated bacteriophage.
  • To classify the phage within existing bacteriophage clusters.

Main Methods:

  • Isolation of bacteriophage from soil using Microbacterium foliorum as the host bacterium.
  • Transmission electron microscopy for morphological analysis.
  • Genome sequencing and bioinformatic analysis to identify genes and determine phylogenetic relationships.

Main Results:

  • The bacteriophage, named DumpQuist, was successfully isolated.
  • Electron microscopy confirmed a podovirus morphology for DumpQuist.
  • DumpQuist possesses a 53,924-bp genome encoding 54 predicted protein-coding genes.
  • Genomic analysis revealed DumpQuist is most similar to phages within the actinobacteriophage cluster EK1.

Conclusions:

  • DumpQuist represents a newly isolated bacteriophage with potential applications in phage therapy or as a research tool.
  • Its classification within the EK1 cluster provides insights into the diversity and evolution of actinobacteriophages.
  • Further research can explore DumpQuist's lytic activity and host range within Microbacterium species.
Keywords:
bacteriophage evolutionbacteriophagesgenome analysis

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