Recent Advances in Bioorthogonal Ligation and Bioconjugation

Area of Science:

• Biochemistry
• Chemical Biology
• Molecular Engineering

Background:

• The development of novel biomolecules with functionalities beyond natural capabilities is a key scientific pursuit.
• Bioconjugation methodologies and reagents are crucial for modifying biomolecules.
• Existing methods have varying scopes and limitations.

Purpose of the Study:

• To provide an overview of recent advances in bioconjugation from 2016 to 2023.
• To highlight novel protein functionalization strategies.
• To discuss new bioorthogonal reagents for modified biomolecules.

Main Methods:

• Review of literature on bioconjugation techniques published between 2016 and 2023.
• Focus on metal-mediated protein functionalization exploiting amino acid reactivity.
• Discussion of novel bioorthogonal reagents for bioconjugation.

Main Results:

• Recent advances have expanded the toolbox for creating functionalized biomolecules.
• Metal-mediated strategies offer selective protein modification.
• New bioorthogonal reagents enable efficient conjugation of modified biomolecules.

Conclusions:

• The field of bioconjugation has seen significant progress, offering enhanced capabilities for biomolecule modification.
• These advances facilitate the creation of engineered biomolecules for diverse applications.
• Continued innovation in bioconjugation is essential for future molecular engineering endeavors.