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Related Concept Videos

Meiosis II02:02

Meiosis II

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Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
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Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
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Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
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Updated: Jul 10, 2025

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
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NUSAP1 regulates mouse oocyte meiotic maturation.

Lina Yu1,2, Na Kong1,3, Yuling Lin2

  • 1Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, China.

Journal of Cellular Biochemistry
|November 22, 2023
PubMed
Summary
This summary is machine-generated.

Nucleolar and spindle-associated protein 1 (NUSAP1) is crucial for mouse oocyte meiosis. Its depletion causes chromosome misalignment and abnormal spindle assembly, highlighting its role in ensuring high-quality oocytes for successful fertilization.

Keywords:
NUSAP1meiosisoocytereproductionspindle

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Accurate chromosome segregation during mouse oocyte meiosis is vital for oocyte quality and fertilization.
  • Spindle-associated proteins regulate spindle dynamics, but the role of NUSAP1 in oocyte meiosis is unknown.
  • NUSAP1 expression significantly increases post-meiotic resumption, suggesting a key role.

Purpose of the Study:

  • To investigate the role of NUSAP1 in mouse oocyte meiosis maturation.
  • To determine NUSAP1 localization and its impact on spindle assembly and chromosome segregation.

Main Methods:

  • Proteomic analysis to identify differentially expressed spindle-associated proteins.
  • Immunofluorescence microscopy to track NUSAP1 localization during oocyte maturation.
  • NUSAP1 depletion via gene editing or RNA interference.
  • Chromosome spread analysis and spindle morphology assessment.
  • RNA-sequencing (RNA-seq) and Gene Ontology (GO) analysis of NUSAP1 interactomes.

Main Results:

  • NUSAP1 localizes to the nucleus in germinal vesicle (GV) oocytes and forms aggregates near spindle poles during meiosis I.
  • NUSAP1 depletion results in chromosome misalignment, aneuploidy, and reduced spindle pole width.
  • RNA-seq reveals suppression of the 'establishment of spindle orientation' pathway in NUSAP1-deficient oocytes.
  • NUSAP1 interactomes are enriched for RNA binding, suggesting a role in mRNA homeostasis and P-body dynamics.

Conclusions:

  • NUSAP1 is essential for proper spindle assembly and chromosome segregation in mouse oocytes.
  • Precise regulation of NUSAP1 expression and localization is critical for maintaining meiotic fidelity.
  • NUSAP1 may regulate oocyte meiosis through RNA-binding activities and influence on P-body components.