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Related Experiment Video

Updated: Jun 18, 2026

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
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Published on: May 18, 2016

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Detecting host responses to microbial stimulation using primary epithelial organoids.

Jette Bornholdt1,2,3, Christina V Müller1, Maria Juul Nielsen1

  • 1Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.

Gut Microbes
|November 22, 2023
PubMed
Summary

Healthy intestinal organoids show distinct responses to probiotic bacteria, unlike cancer cell lines. This research highlights organoids as a better model for studying host-microbe interactions.

Keywords:
Intestinal organoidsbacterial–epithelial interactionsintestinal epitheliummicrobiomeprobiotics

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Area of Science:

  • Microbiology
  • Gastroenterology
  • Cell Biology

Background:

  • The intestinal epithelium interacts with luminal microbes.
  • Cancer-derived cell lines (e.g., Caco-2) are traditionally used to study these interactions.
  • It remains unclear if these cell lines accurately represent normal epithelial responses and strain-specific microbial effects.

Purpose of the Study:

  • To investigate microbial responses in normal intestinal epithelium using organoids.
  • To compare responses between healthy organoids and Caco-2 cell lines.
  • To identify common and strain-specific microbial interactions.

Main Methods:

  • Derived intestinal organoids from healthy individuals (n=9).
  • Cultured organoids on a laminin matrix to induce differentiation and apical exposure.
  • Exposed differentiated organoids to probiotic bacterial strains (BB-12®, LGG®, DSM33361, Bif195).
  • Performed parallel experiments using Caco-2 cell lines.

Main Results:

  • Healthy intestinal organoids exhibited robust acute responses to various probiotic strains.
  • Both common and strain-specific responses were observed across individuals.
  • Caco-2 cell lines showed no acute response to the tested probiotics.
  • Organoid models revealed individual variability in microbial responses.

Conclusions:

  • Primary intestinal organoids from healthy individuals are a valuable model for studying host-microbe interactions.
  • Organoids provide a more accurate representation of normal epithelial responses compared to Caco-2 cells.
  • This approach enhances understanding of individual variations in host-microbe dynamics and microbial strain specificity.