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Smartphone-integrated paper-based biosensor for sensitive fluorometric ethanol quantification.

Mallesh Santhosh1, Tusan Park2,3

  • 1Smart Agriculture Innovation Center, Kyungpook National University, Daegu, Republic of Korea.

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|November 22, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a portable, instrument-free method using fluorometric paper-based analytical devices (fPADs) and smartphones for accurate ethanol quantification. The developed fPADs offer high sensitivity and stability, making them ideal for on-site food quality control.

Keywords:
Ethanol biosensorPaper-based assayPortable fluorescence detectionSmartphone-based sensorWine analysis

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Materials Science

Background:

  • Accurate ethanol quantification is crucial for food and beverage quality control.
  • Existing methods often require expensive instrumentation and laboratory settings.
  • There is a need for portable, cost-effective, and user-friendly analytical tools.

Purpose of the Study:

  • To develop and validate a novel fluorometric paper-based analytical device (fPAD) for instrument-free ethanol quantification.
  • To integrate fPADs with smartphone technology for enhanced portability and accessibility.
  • To assess the performance, stability, and applicability of the developed fPADs in real-world samples.

Main Methods:

  • Immobilization of NAD+-dependent alcohol dehydrogenase on chitosan-modified paper as a bio-recognition element.
  • Utilizing enzymatically generated NADH as a fluorescent probe for ethanol detection.
  • Development of a 3D-printed imaging setup with UV-LED for rapid fluorescent image capture.
  • Validation of fPADs using standard ethanol solutions and analysis of various wine samples.

Main Results:

  • The developed fPADs demonstrated high sensitivity for ethanol detection with a linear range from 17 µM to 8.75 mM (R² = 0.99).
  • Successful quantification of ethanol in red, white, rose, and sparkling wine samples was achieved.
  • The fPADs exhibited excellent reproducibility and enzyme stability, retaining activity for at least 28 days.

Conclusions:

  • The proposed fPADs offer a facile, affordable, and portable solution for on-site ethanol quantification.
  • This technology is suitable for food quality control applications, enabling rapid and accurate analysis.
  • Integration with smartphone technology enhances the accessibility and practicality of the analytical device.