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Jagged1 contained in MSC-derived small extracellular vesicles promotes squamous differentiation of cervical cancer by

Weizhao Li1,2, Xunzhi Zhang3, Tianshun Gao1

  • 1Department of Gynecology, Pelvic Floor Disorders Center, Scientific Research Center, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, China.

Journal of Cancer Research and Clinical Oncology
|November 23, 2023
PubMed
Abstract

Insights

Mesenchymal stem cell-derived small extracellular vesicles (MSC-sEV) show promise for cervical cancer treatment by activating the NOTCH pathway, promoting squamous differentiation, and inhibiting cancer cell growth and migration.

Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • Cervical cancer is a significant global health concern for women, necessitating novel therapeutic strategies.
  • Current treatments face limitations, driving research into innovative approaches like stem cell-derived therapies.

Purpose of the Study:

  • To investigate the therapeutic potential of small extracellular vesicles derived from human umbilical cord mesenchymal stem cells (MSC-sEV) in cervical cancer.
  • To elucidate the effects of MSC-sEV on cervical cancer cell lines, focusing on squamous differentiation and the NOTCH signaling pathway.

Main Methods:

  • Extraction and identification of MSC-sEV from human umbilical cord-derived mesenchymal stem cells.
  • In vitro assessment of MSC-sEV effects on CaSki (cervical cancer) and HaCaT (keratinocyte) cell lines.
  • Analysis of NOTCH pathway activation, cell growth, proliferation, migration, and squamous differentiation.

Main Results:

  • MSC-sEV, specifically Jagged1 protein, bind to NOTCH1 on cervical cancer cells, activating the NOTCH pathway.
  • MSC-sEV promote squamous differentiation in CaSki cells, inhibiting their growth, proliferation, and migration.
  • MSC-sEV activate the NOTCH pathway in HaCaT cells, enhancing their viability.

Conclusions:

  • MSC-sEV represent a potential novel therapeutic agent for cervical cancer by activating the NOTCH pathway.
  • The mechanism involves promoting squamous differentiation and inhibiting the growth, proliferation, and migration of cervical cancer cells.
Keywords:
Cervical cancerDifferentiation therapyMSC-sEVMesenchymal stem cellsNOTCH pathway

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