A Simple Selective Probe for Lysine Detection in Tablets, Food Samples and Cells

Area of Science:

• Analytical Chemistry
• Organic Chemistry
• Biochemistry

Background:

• Amino acid detection is crucial for biological and medical research.
• Developing selective and sensitive probes is essential for accurate quantification.
• Lysine plays vital roles in protein structure and function.

Purpose of the Study:

• To design and synthesize a novel probe for selective lysine detection.
• To evaluate the probe's sensing capabilities and binding affinity.
• To demonstrate the probe's applicability in complex matrices and biological systems.

Main Methods:

• Synthesis of the novel probe ITQ.
• Spectroscopic analysis (absorption and emission) for sensing behavior.
• Binding studies to determine affinity and stoichiometry.
• Application in real samples and live cell imaging.

Main Results:

• Successful synthesis of the ITQ probe.
• Selective absorption and emission spectral responses to lysine.
• High binding affinity for lysine (1.4 × 10^4 M^-1) at a 1:2 stoichiometry.
• Detection of lysine in nanomolar concentrations within a pH range of 4-11.
• Successful detection in tablets, food samples (avocado, soybean, pork), and HeLa cells.

Conclusions:

• The novel ITQ probe demonstrates excellent selectivity and sensitivity for lysine detection.
• ITQ is a promising tool for quantifying lysine in diverse biological and real-world samples.
• The probe's performance across various matrices highlights its potential for practical applications.