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Updated: Jul 10, 2025

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The relationship between postmortem interval and protein changes in mice.

Wangping Zhang1, Kaikai Dong1, Jing Sun2

  • 1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

Journal of Forensic and Legal Medicine
|November 23, 2023
PubMed
Summary
This summary is machine-generated.

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Estimating time since death (postmortem interval) is crucial. This study identifies specific protein changes in mouse liver and spleen that correlate with postmortem interval, aiding forensic analysis.

Area of Science:

  • Forensic Science
  • Biochemistry
  • Proteomics

Background:

  • Accurate postmortem interval (PMI) estimation is vital for forensic pathology.
  • While protein degradation correlates with PMI, specific biomarkers remain elusive.
  • Previous research indicates potential protein markers for time of death determination.

Purpose of the Study:

  • To investigate protein expression changes in mouse liver and spleen over time postmortem.
  • To identify specific proteins that can serve as reliable indicators for PMI estimation.
  • To explore the utility of proteomic techniques in forensic science for PMI determination.

Main Methods:

  • Measurement of total protein content in mouse liver and spleen at various postmortem times.
  • Analysis of β-actin degradation in the liver.
Keywords:
Forensic pathologyMouse liverPostmortem intervalProtein degradationProteomics

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  • Proteomic analysis using Two-dimensional electrophoresis and peptide mass fingerprinting (via MALDI-TOF/MS) to identify differentially expressed proteins.

    • Main Results:

    • Total protein content in liver and spleen showed a negative correlation with PMI.
    • β-actin degradation in the liver demonstrated a positive correlation with PMI.
    • Seven proteins in the liver showed significant changes in expression; six were identified, including SBP2, ENOA, ALDH2 (decreased) and 3HAO, TPIS, CATA (unchanged).

    Conclusions:

    • Specific protein alterations in the liver are linked to postmortem interval.
    • Proteins like SBP2, ENOA, and ALDH2 show degradation patterns useful for PMI estimation.
    • Unchanged proteins (3HAO, TPIS, CATA) may serve as internal references for more accurate PMI determination in future forensic applications.