Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Global adaptation to climate change in the twilight zone revealed by shared signals of selection in mesopelagic lanternfishes.

bioRxiv : the preprint server for biology·2026

Same author

The Spatiotemporal Structure of Neural Activity in Motor Cortex during Reaching.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same author

Functional Clusters for Shape, Texture, and Motion Encoding in Macaque V2.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Same author

Platelet Hyaluronan Synthase 3 Regulates Thrombin Signaling and Adhesion to Fibrinogen Under Venous Shear.

Proteoglycan research·2026

Same author

Successful Treatment of Trichodectes canis in a Dog with a 1-Month Formulation of Fluralaner.

Journal of the American Animal Hospital Association·2025

Same author

Longitudinal multitask wireless electrocorticography data from two fully implanted nonhuman primates.

Scientific data·2025

Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026

Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026

Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026

Same journal

Methods for Measuring Neural Activity During Voluntary Wheel Running.

Journal of neuroscience methods·2026

Same journal

Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

Journal of neuroscience methods·2026

Same journal

Rapid generation of human sensory neurons from iPSC for modeling of peripheral neuropathies.

Journal of neuroscience methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 10, 2025

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Published on: October 19, 2011

Inserting a Neuropixels probe into awake monkey cortex: two probes, two methods.

Tomoyuki Namima¹, Erin Kempkes², Bob Smith³

¹Department of Biological Structure and Washington National Primate Research Center, University of Washington, Seattle, WA 98195, USA; Graduate School of Frontier Biosciences, Osaka University, and Center for Information and Neural Networks, National Institute of Information and Communications Technology, Suita, Osaka 565-0871, Japan.

Journal of Neuroscience Methods

|November 23, 2023

Summary

This summary is machine-generated.

Researchers developed novel methods for inserting Neuropixels probes into the primate brain, overcoming challenges with the thicker dura. These techniques enable reliable, long-term neurophysiological recordings in awake macaque monkeys.

Keywords:

Chronic recording chamber High-density silicon probe Multi-contact linear probes Nonhuman primate neurophysiology

More Related Videos

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Published on: March 4, 2012

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Published on: June 13, 2017

Related Experiment Videos

Last Updated: Jul 10, 2025

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Published on: October 19, 2011

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Published on: March 4, 2012

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Published on: June 13, 2017

Area of Science:

Neuroscience
Primate Physiology
Neurotechnology

Background:

Neuropixels probes are essential for rodent neurophysiology.
Primate dura presents a significant barrier to probe insertion.
Existing methods are insufficient for primate brain recordings.

Purpose of the Study:

To develop and validate methods for acute Neuropixels probe insertion in awake macaque monkeys.
To overcome challenges associated with primate dura mater thickness.
To enable high-yield, long-term neurophysiological recordings in non-human primates.

Main Methods:

Developed a dural-eyelet method for fine Neuropixels 1.0 (IMEC) probes.
Created an artificial dura system for thicker Neuropixels NP1010 probes.
Implemented surgical preparation and probe insertion/removal techniques for awake macaques.

Main Results:

Successfully inserted Neuropixels probes in 3 animals across 7 recording chambers.
Achieved consistent, high-yield recordings over several months.
Demonstrated the efficacy of both the dural-eyelet and artificial dura methods.

Conclusions:

The developed methods facilitate reliable Neuropixels probe insertion in primates.
These techniques are valuable for advancing primate neurophysiology research.
The methods support hardware, surgical preparation, and probe removal strategies for primate studies.