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Updated: Jul 10, 2025

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
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Functional analysis of transgenic cry1Ah-1 maize.

Fan-Hao Wang1, Li-Yuan Han1, Qing-Ping Jiang1

  • 1College of Life Sciences, Jilin Agricultural University, Changchun, 130118, China; Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Changchun, 130118, China.

Microbial Pathogenesis
|November 23, 2023
PubMed
Summary

Genetically modified maize with the cry1Ah-1 gene offers robust protection against corn borers. This enhanced maize variety demonstrates high insecticidal activity and a promising outlook for commercialization.

Keywords:
Insect resistanceMaizecry1Ah-1

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Area of Science:

  • Agricultural Science
  • Genetics
  • Entomology

Background:

  • Maize yield and quality are significantly impacted by insect pests.
  • Developing insect-resistant maize is crucial for global food security.

Purpose of the Study:

  • To develop insect-resistant maize by introducing a mutated cry1Ah gene.
  • To evaluate the efficacy of transgenic maize against corn borers.

Main Methods:

  • Mutagenesis of the cry1Ah gene using error-prone PCR.
  • Agrobacterium-mediated transformation of maize inbred line GSH9901 with the mutant cry1Ah-1 gene.
  • Molecular detection (PCR, qRT-PCR, immunoassay, Western blot, ELISA) and biological activity assays.

Main Results:

  • Transgenic maize plants (T2 generation) successfully expressed the Cry1Ah-1 gene and protein.
  • High relative expression levels of Cry1Ah-1 were observed in OE1 and OE3 lines.
  • 100% mortality rate of corn borers in laboratory tests and high insecticidal activity in field trials were achieved.

Conclusions:

  • Transgenic cry1Ah-1 maize exhibits strong insecticidal effects against corn borers.
  • The developed transgenic maize shows significant potential for commercial application in pest management.