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Functional analysis of transgenic cry1Ah-1 maize
Fan-Hao Wang1, Li-Yuan Han1, Qing-Ping Jiang1
1College of Life Sciences, Jilin Agricultural University, Changchun, 130118, China; Joint International Research Laboratory of Modern Agricultural Technology, Ministry of Education, Changchun, 130118, China.
Genetically modified maize with the cry1Ah-1 gene offers robust protection against corn borers. This enhanced maize variety demonstrates high insecticidal activity and a promising outlook for commercialization.
Area of Science:
- Agricultural Science
- Genetics
- Entomology
Background:
- Maize yield and quality are significantly impacted by insect pests.
- Developing insect-resistant maize is crucial for global food security.
Purpose of the Study:
- To develop insect-resistant maize by introducing a mutated cry1Ah gene.
- To evaluate the efficacy of transgenic maize against corn borers.
Main Methods:
- Mutagenesis of the cry1Ah gene using error-prone PCR.
- Agrobacterium-mediated transformation of maize inbred line GSH9901 with the mutant cry1Ah-1 gene.
- Molecular detection (PCR, qRT-PCR, immunoassay, Western blot, ELISA) and biological activity assays.
Main Results:
- Transgenic maize plants (T2 generation) successfully expressed the Cry1Ah-1 gene and protein.
- High relative expression levels of Cry1Ah-1 were observed in OE1 and OE3 lines.
- 100% mortality rate of corn borers in laboratory tests and high insecticidal activity in field trials were achieved.
Conclusions:
- Transgenic cry1Ah-1 maize exhibits strong insecticidal effects against corn borers.
- The developed transgenic maize shows significant potential for commercial application in pest management.
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