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Updated: Aug 4, 2026

The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Direct evidence of the ability of Pseudomonas aeruginosa and E. coli SulA to dimerize
N A Rumyantseva1, D M Golofeeva1, A V Shabalina2
1Peter the Great St.Petersburg Polytechnic University, Polytechnicheskaya 29, Saint-Petersburg, 195251, Russia.
Abstract:
The SulA protein of Escherichia coli and related bacteria interacts directly with FtsZ, blocking cell division by disrupting Z-ring formation, yet the precise mechanism remains not fully understood. Previous demonstrations of Pseudomonas aeruginosa SulA's dimerization capability were confined to X-ray crystallography and lacked confirmation under in vivo conditions. Additionally, uncertainty persisted regarding the dimerization potential of E. coli's SulA protein. This paper employs a bacterial two-hybrid system to establish that both P. aeruginosa and E. coli SulA proteins indeed possess the capacity for dimerization.
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