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Related Experiment Video

Updated: Jul 10, 2025

Picoinjection of Microfluidic Drops Without Metal Electrodes

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

pH Regulator on Digital Microfluidics with Pico-Dosing Technique.

Haoran Li¹, Tao Peng², Yunlong Zhong³

¹The State Key Laboratory of Analog and Mixed-Signal VLSI, Institute of Microelectronics, University of Macau, Macau 999078, China.

|November 24, 2023

Summary

This study introduces a flexible digital microfluidic (DMF) platform for real-time pH control in cell-based experiments. Pico-dosing technology precisely adjusts pH, enhancing on-chip biological research flexibility.

Keywords:

digital microfluidic pH regulator pico-dosing

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Last Updated: Jul 10, 2025

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Area of Science:

Microfluidics
Biotechnology
Analytical Chemistry

Background:

Real-time pH control is essential for microfluidic cell-based experiments but remains challenging.
Existing methods lack flexibility and precision for on-chip applications.

Purpose of the Study:

To develop a flexible and precise on-chip pH regulator for digital microfluidic (DMF) platforms.
To enable real-time pH monitoring and adjustment for improved biological experiments.

Main Methods:

Utilized pico-dosing technology to generate and transfer satellite droplets for precise alkali/acid delivery.
Developed an ImageJ-based image analysis method for accurate delivered volume calculation.
Implemented an on-chip colorimetric method with an acid-base indicator for pH determination.

Main Results:

Demonstrated precise control over sample pH using the pico-dosing system.
Showed consistency between calculated delivered volumes and measured pH values.
Validated the effectiveness of the on-chip colorimetric pH sensing method.

Conclusions:

The developed DMF platform offers a flexible and easy-to-control solution for real-time pH regulation.
This technology enhances the precision and reliability of on-chip biological experiments.
The approach simplifies complex pH adjustments, paving the way for advanced microfluidic applications.