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Continuous Real-Time Detection of Serotonin Using an Aptamer-Based Electrochemical Biosensor.

Habib M N Ahmad1, Arturo Andrade2,3, Edward Song1

  • 1Department of Electrical & Computer Engineering, University of New Hampshire, Durham, NH 03824, USA.

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This study introduces a novel electrochemical biosensor for continuous, real-time monitoring of serotonin (5-HT). The highly sensitive and selective aptamer-based sensor offers rapid detection, crucial for understanding neurological functions and mental health.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Serotonin (5-HT) is vital for neuronal functions, but its depletion is linked to mental health disorders.
  • Detecting serotonin is challenging due to its rapid release/clearance, low concentrations, and interfering substances.

Purpose of the Study:

  • To develop a highly sensitive and selective electrochemical aptamer-based biosensing platform for continuous serotonin monitoring.
  • To enable real-time detection of serotonin in biological samples.

Main Methods:

  • Utilized an electrochemical aptamer-based biosensor.
  • Employed a single-frequency electrochemical impedance spectroscopy (EIS) technique for continuous monitoring.
  • Tested sensor performance in continuous sample fluid flow.

Main Results:

  • Achieved a response time of approximately 1 minute to concentration changes.
  • Demonstrated a detection limit (LOD) of 5.6 nM for serotonin in the 25-150 nM range.
  • Exhibited high selectivity against interfering compounds like dopamine and norepinephrine.

Conclusions:

  • The developed aptasensor provides a robust platform for continuous, real-time serotonin detection with high sensitivity and selectivity.
  • This technology holds potential for use in organ-on-a-chip and brain-on-a-chip systems for neurological research.