Transcriptome Changes in Glioma Cells upon Infection with the Oncolytic Virus VV-GMCSF-Lact
Dmitriy V Semenov1, Natalia S Vasileva1, Maya A Dymova1
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentyev Avenue, 8, 630090 Novosibirsk, Russia.
Cells
|November 24, 2023
Summary
This study explored how VV-GMCSF-Lact oncolytic virus impacts glioma cells. It identified key gene expression changes, revealing molecular markers that influence cancer virotherapy effectiveness.
Area of Science:
- Oncolytic Virotherapy
- Molecular Biology
- Cancer Research
Background:
- Oncolytic virotherapy utilizes viruses to selectively target and destroy cancer cells.
- Glioma, a type of brain tumor, presents significant treatment challenges.
- Recombinant vaccinia virus VV-GMCSF-Lact is a novel agent explored for solid tumor treatment.
Purpose of the Study:
- To investigate the molecular effects of VV-GMCSF-Lact on human glioma cells.
- To identify gene expression patterns associated with sensitivity and resistance to VV-GMCSF-Lact.
- To uncover potential biomarkers for enhancing oncolytic virotherapy efficacy.
Main Methods:
- Transcriptome analysis of immortalized and patient-derived glioma cells post-infection with VV-GMCSF-Lact.
- Comparison of gene expression profiles in infected glioma cells versus non-malignant brain cells.
- Correlation analysis between gene expression levels and cellular sensitivity to viral cytotoxicity.
Main Results:
- VV-GMCSF-Lact infection commonly activated histone genes and upregulated inflammatory/interferon-gamma response pathways.
- Genes involved in cell cycle progression were downregulated post-infection.
- Upregulation of Golgi vesicle and protein transport genes correlated with reduced sensitivity, while mRNA splicing gene upregulation indicated increased sensitivity.
Conclusions:
- VV-GMCSF-Lact influences glioma cell biology by modulating key cellular processes.
- Specific gene expression profiles can predict glioma cell sensitivity to VV-GMCSF-Lact.
- Identifying these molecular markers is crucial for optimizing oncolytic virotherapy strategies in glioma treatment.
Keywords:
VV-GMCSF-Lactdifferentially expressed genesglioblastomagliomanext-generation sequencingoncolytic viruspatient-derived cell culturestranscriptomevaccinia virusvirotherapyMore Related Videos
Related Concept Videos
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...