Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

The Retinoblastoma Gene

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Receptor for Advanced Glycation End Products (RAGE) Ligand Axis as a Mediator of Inflammation and Oxidative Stress in Cancer: Implications for Cancer Progression and Therapeutic Targeting.

Current pharmaceutical design·2026

Same author

Development, image interpretation, clinical experience, and applications of optical coherence tomography in neurointerventional surgery.

Journal of neurointerventional surgery·2026

Same author

A multicenter retrospective cohort study showing that bridging thrombolysis does not achieve better outcomes compared to direct mechanical thrombectomy in stroke due to internal carotid artery occlusion.

Neuroradiology·2026

Same author

Thrombectomy for distal medium vessel occlusion stroke: Combined vs. single-device techniques - A systematic review and meta-analysis.

Frontiers in stroke·2026

Same author

Direct dotterising or angioplasty of acute stroke due to tandem atherosclerotic occlusions.

Frontiers in stroke·2026

Same author

Post-marketing study of patients to evaluate NIMBUS revascularization effectiveness with challenging occlusions (SPERO): study results.

Journal of neurointerventional surgery·2026

Search research articles

Related Experiment Video

Updated: Jul 10, 2025

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer.

Lubna M Mehdawi¹, Souvik Ghatak¹, Payel Chakraborty¹

¹Cell and Experimental Pathology, Department of Translational Medicine, Lund University, Skåne University Hospital, SE 214 28 Malmö, Sweden.

|November 24, 2023

Summary

WNT5A signaling, including the peptide Foxy5, suppresses colon cancer growth by reducing LGR5/RSPO3 and β-catenin activity. This inhibits cancer stemness and VEGFA, offering new therapeutic avenues.

Keywords:

Foxy5 LGR5 R-spondin3 VEGFA WNT/β-catenin signaling WNT5A

More Related Videos

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal

Published on: October 11, 2019

Related Experiment Videos

Last Updated: Jul 10, 2025

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal

Analyzing Tumor Gene Expression Factors with the CorExplorer Web Portal

Published on: October 11, 2019

Area of Science:

Oncology
Molecular Biology
Cancer Signaling Pathways

Background:

WNT/β-catenin signaling is crucial for colon cancer progression.
WNT5A and Foxy5 are known to inhibit β-catenin signaling through unclear mechanisms.

Purpose of the Study:

Investigate WNT5A's impact on LGR5, RSPO3, β-catenin activity, and VEGFA in colon cancer.
Determine the relationship between WNT5A and colon cancer stemness markers like DCLK1.

Main Methods:

Analysis of colon cancer patient cohorts (immunohistochemistry, qRT-PCR).
In vitro and in vivo experiments using colon cancer cell lines (Western blotting, RT-PCR, assays, immunofluorescence).

Main Results:

WNT5A expression negatively correlated with LGR5/RSPO3 and DCLK1 in patient cohorts.
WNT5A signaling suppressed β-catenin activity, LGR5, RSPO3, VEGFA, and cancer stemness markers.
Foxy5 peptide mimicked WNT5A's inhibitory effects.

Conclusions:

WNT5A and Foxy5 reduce LGR5/RSPO3 expression and β-catenin activity.
This inhibition impacts colon cancer stemness and VEGFA expression.
WNT5A/Foxy5 represent potential therapeutic strategies for colon cancer.