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Related Experiment Video

Updated: Jul 10, 2025

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Published on: December 16, 2019

High Sensitivity Singlet Oxygen Luminescence Sensor Using Computational Spectroscopy and Solid-State Detector.

Tiffany C Yu¹, Steve J Davis¹, Mark T Scimone¹

¹Physical Sciences Inc., Andover, MA 01810, USA.

Diagnostics (Basel, Switzerland)

|November 24, 2023

Summary

This summary is machine-generated.

This study introduces a new computational spectroscopy method for highly sensitive singlet oxygen measurement during photodynamic therapy (PDT) and UV exposure. This technique can guide PDT treatments and aid in developing better sunscreens for skin cancer prevention.

Keywords:

UV skin irradiation luminescence spectroscopy photodynamic therapy singlet oxygen skin cancer

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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

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Published on: June 28, 2016

Area of Science:

Biomedical Optics
Photochemistry
Spectroscopy

Background:

Photodynamic therapy (PDT) relies on generating singlet oxygen, a key cytotoxic agent.
Accurate measurement of singlet oxygen is crucial for optimizing PDT efficacy and safety.
Current methods for singlet oxygen detection face limitations in sensitivity and cost.

Purpose of the Study:

To develop a high-sensitivity measurement technique for singlet oxygen luminescence.
To enable accurate quantification of singlet oxygen generated during PDT and UV irradiation on skin.
To explore the application of this technology as a dosimeter for PDT and in sunscreen development.

Main Methods:

Utilized a computational spectroscopy (CS) approach with a solid-state InGaAs photodiode detector.
Achieved high photon detection efficiency and spectral resolution.
Enabled accurate subtraction of photosensitizer fluorescence without time-gating.

Main Results:

Demonstrated high sensitivity in detecting singlet oxygen luminescence.
Showed correlation between measured singlet oxygen and PDT treatment efficacy in vivo and ex vivo.
Validated the relationship between singlet oxygen levels and UV illumination intensity on skin.

Conclusions:

The CS technique offers a cost-effective and robust method for singlet oxygen measurement.
The technology shows potential as a dosimeter for guiding PDT treatments.
This approach can support the development of advanced sunscreen formulations for skin cancer prevention.